Experimental Design Sample Work
Effective methods for metabarcoding fungi: from experimental design to outcomes
Abstract:
The advancement of high-throughput Sequencing (HTS) technology has dramatically improved our ability to detect fungi and reveal their ecological research importance in a wide range of habitats.
Overview:
From experimental design to molecular and computational analyses, we present an overview of current best practices in the metabarcoding investigation of fungal communities. We show that operational taxonomic units (OTUs) outperform amplified sequence variants (ASVs) in recovering fungal diversity, especially for lengthy markers, by reanalyzing published data collection sets. Furthermore, as compared to the ITS2 sub region, examination of the whole ITS region allows for more precise taxonomic placement of fungus and other eukaryotes. Finally, we show that specialized strategies for analyzing compositional data yield more trustworthy estimations of community structure alterations.
Conclusion:
We conclude that fungus metabarcoding investigations are especially promising for integrating fungi into the complete microbiome and larger systematic ecosystem functioning environment, recovering new fungal lineages and ancient species, and barcoding old specimens, including type material.
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Tags:
Experimental Research | Genome Sequencing | Bioinformatics | Statistical Analyses |
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